RBRC05641

Information1

Image
BRC No.RBRC05641
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pla2g4a<tm1Tksh>
Former Common nameB6-Pla2g4a KO, cPLA2a KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Naonori Uozumi and Takao Shimizu, Faculty of Medicine, University of Tokyo in 1997. E14.1 ES cells derived from 129P2/PlaHsd were used. The mutant mice were backcrossed to C57BL/6JJcl.
Strain descriptionPla2g4a gene knockout mice. A part of the exon encoding the lipase consensus motif and serine 288 of Pla2g4a was replaced with a PGK-neo cassette. Homozygous mutant mice show reduced allergic and autoimmune reactions and females of homozygous are infertile (showing abnormal parturition and decreased litter size).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant females are infertile.
ReferencesNature, 390, 618-622 (1997). 9403692
Nature, 390, 622-625 (1997). 9403693

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pla2g4aphospholipase A2, group IVA (cytosolic, calcium-dependent)1Pla2g4a<tm1Tksh>targeted mutation 1, Takao Shimizu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), Mouse Phosphoglycerate kinase polyA
Research applicationImmunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 390, 618-622 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakao Shimizu (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data