Strain Data Sheet



BRC No.RBRC05638
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nov<tm1Hko>
Former Common nameCCN3 KO, B6-Nov KO, B6;129-Nov<tm1Hko>, B6;129-Nov<tm1Hko>/HkoRbrc
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used for the generation of knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionNov (CCN3) gene knockout mice. Exons 1, 2 and 3 of Nov gene were replaced with a neomycin selection cassette. Homozygous mutant mice show no obvious abnormality. Homozygous mutant mice exhibit enhanced neointimal hyperplasia in vascular smooth muscles induced by endothelial injury.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesArterioscler. Thromb. Vasc. Biol., 30, 675-682 (2010). 20139355

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ccn3nephroblastoma overexpressed gene15Ccn3<tm1Hko>targeted mutation 1, Haruhiko KosekiNov

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, mouse Nov genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data