Strain Data Sheet

RBRC05637

Information1

Image
BRC No.RBRC05637
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;129S2-Igsf8<tm1.1Osb>
Former Common nameIgsf8 floxed
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice (B6). C57BL/6, DBA/2 and 129 mixed background.
Strain descriptionIgsf8 floxed mice. Exons 3 to 5 were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:B6D2F1]
ReferencesFertil. Steril., 98(2):465-470 (2012). 22609062

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Igsf8immunoglobulin superfamily, member 81Igsf8<tm1.1Osb>targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Information2

Donor DNAP1 phage loxP sites, yeast FRT (flipase recombination target) site, mouse Igsf8 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Fertil. Steril., 98(2):465-470 (2012).
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Aug 2015
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Jimbo K, Nakajima-Takagi Y, Ito T, Koide S, Nannya Y, Iwama A, Tojo A, Konuma T.
Immunoglobulin superfamily member 8 maintains myeloid leukemia stem cells through inhibition of β-catenin degradation.
Leukemia (2022) 35418614