RBRC05631

Information1

Image
BRC No.RBRC05631
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Nin<tm1a(EUCOMM)Hmgu>/B10
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2017/04/17Room:3-DRack:D
2017/02/20Room:3-DRack:D
2016/12/19Room:3-DRack:D
2016/10/17Room:3-DRack:D
2016/08/22Room:3-DRack:D
2016/06/20Room:3-DRack:D
2016/04/18Room:3-DRack:D
2016/02/15Room:3-DRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ninninein12Nin<tm1a(EUCOMM)Hmgu>targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)12En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12human beta-actin promoter

Information2

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Nin genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data