Strain Data Sheet


Strain Information

BRC No.RBRC05604
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Stxbp1<tm1Ksak>
Former Common nameMunc18-1 floxed, B6-Stxbp1 floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Strain developmentDeveloped by Kenji Sakimura, Brain Research Institute, Niigata University in 2008. C57BL/6N background.
Strain descriptionMunc18-1 (Stxbp1) gene conditional knockout mice. Exon 3 of Stxbp1 was flanked by loxP sites. Conditional Munc18-1 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous null knockout mice die within 24 hrs after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesBiochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751
Hum. Mol. Genet., 26(24):4961-4974 (2017). 29040524

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Stxbp1syntaxin binding protein 12Stxbp1<tm1Ksak>targeted mutation 1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNAYeast FRT (flippase recombination target) site, P1 Phage LoxP sites, mouse Stxbp1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgement to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for scientific research for publishing paper(s). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results obtained by using the BIOLOGICAL RESOURCE. For the use of BIOLOGICAL RESOURCE by a for-profit institutions, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data