RBRC05601

Information1

Image
BRC No.RBRC05601
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129(Cg)-Rab7<tm1.2Ywd>
Former Common nameRab7 null, B6-rAB7 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An Rab7 targeted allele, containing a loxP site in upstream of exon 2 and a RT-loxP-Neo-FRT-loxP cassette in downstream of exon 13, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to Rab7- (Rab7<tm1.1Ywd>) by crossing with FLP and Cre mice (B6;SJL-Tg(ACTFLPe)9205Dym/J and FVB/N-Tg(EIIa-cre)C5379Lmgd/J). The mutant mice were cross to C57BL/6.
Strain descriptionRab7 gene knockout mice. Exons 2 and 3 of the Rab7 gene were removed. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesDev. Cell, 22, 1163-1175 (2012). 22698281
Nat. Commun., 3, 1071 (2012). 22990867

Health Report

Examination Date / Room / Rack2013/11/25Room:4-ARack:B
2013/10/25Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rab7RAB7, member RAS oncogene family6Rab7 <tm1.2Ywd>targeted mutation 1.2, Yoh Wada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAEscherichia coli Tn7 Right border and left border, Bacteriophage P1 LoxP site, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Rab7 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorYoh Wada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data