RBRC05598

Information1

Image
BRC No.RBRC05598
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129(Cg)-Vps41<tm1Ywd>
Former Common namemVam2 floxed, B6-Vps41 floxed
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An m Vam2-floxNeo allele, containing the FRT-loxP-Npt-FRT-loxP in intron 13, and the third loxP sequence in intron 17, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to mVam2-floxed (Vps41<tm1Ywd>) by crossing with FLP mice (B6;SJL-Tg(ACTFLPe)9205Dym/J). The mutant mice were cross to C57BL/6.
Strain descriptionmVam2 (Vps41) floxed mice. Exons 14 to 18 of the mVam2 gene were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesDev. Cell, 22, 1163-1175 (2012). 22698281
Nucleic Acids Res., 33, e52 (2005). 15784610

Health Report

Examination Date / Room / Rack2014/02/18Room:3-DRack:L
2014/02/05Room:3-DRack:L
2014/02/03Room:3-DRack:L
2014/01/08Room:3-DRack:L
2013/12/16Room:3-DRack:L
2013/12/16Room:3-DRack:L
2013/10/21Room:3-DRack:L
2013/08/26Room:3-DRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vps41vacuolar protein sorting 41 (yeast)13Vps41 <tm1Ywd>targeted mutation 1, Yoh Wada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Information2

Donor DNAEscherichia coli Tn7 Right border and left border, Bacteriophage P1 loxP sites, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Vps41 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorYoh Wada (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data