Strain Data Sheet
RBRC05584
Information1 | |
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| Image | |
| BRC No. | RBRC05584 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Ikzf5<tm1(KOMP)Wtsi>/C06 |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
| Background strain | C57BL/6NTac |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Mamm. Genome, 23(9-10), 580-586 (2012). 22968824 Nature Methods 6, 493-495 (2009). 19525957 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ikzf5IKAROS family zinc finger 57Ikzf5<tm1(KOMP)Wtsi>targeted mutation 1, Wellcome Trust Sanger Institute Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)7En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)7internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;human beta-actin promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal7 |
Information2 | |
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| Donor DNA | yeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Ikzf5 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The mice were originally created by the National Institutes of Health (NIH) Knock-Out Mouse Program (KOMP). The RECIPIENT is free to publish, present, display, disclose or seek patent or other intellectual property protection on any inventions arising from the use of the KOMP Materials, with no restrictions or obligations of any kind, so long as the RECIPIENT uses reasonable efforts to credit or otherwise acknowledge the KOMP as the creator of the KOMP Materials. The RECIPIENT shall use the following acknowledgement phrase in any resulting publication: The Ikzf5 KO mice were obtained from the RIKEN BioResource Center which generated the The Ikzf5 KO mice using knockout ES cells provided by the KOMP Repository and is a member of the International Mouse Phenotyping Consortium (IMPC). Associated primary phenotypic information may be found at www.mousephenotype.org. When details of the alleles have been published, the RECIPIENT shall include the following references in publications: Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling M-C, Bottomley J, Brown SD, Burger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, et al. (2012) The mammalian gene function resource: the international knockout mouse consortium. Mamm. Genome, 23(9-10), 580-586. Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A & Skarnes WC (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature methods, 6(7), 493-495. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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