RBRC05584

Information1

Image
BRC No.RBRC05584
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ikzf5<tm1(KOMP)Wtsi>/C06
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2019/03/11Room:2-検Rack:C
2019/01/15Room:2-検Rack:C
2018/11/12Room:2-検Rack:C
2018/09/10Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ikzf5IKAROS family zinc finger 57Ikzf5<tm1(KOMP)Wtsi>targeted mutation 1, Wellcome Trust Sanger Institute

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)7En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7human beta-actin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal7

Information2

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Ikzf5 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe mice were originally created by the National Institutes of Health (NIH) Knock-Out Mouse Program (KOMP). The RECIPIENT is free to publish, present, display, disclose or seek patent or other intellectual property protection on any inventions arising from the use of the KOMP Materials, with no restrictions or obligations of any kind, so long as the RECIPIENT uses reasonable efforts to credit or otherwise acknowledge the KOMP as the creator of the KOMP Materials.
The RECIPIENT shall use the following acknowledgement phrase in any resulting publication:
The Ikzf5 KO mice were obtained from the RIKEN BioResource Center which generated the The Ikzf5 KO mice using knockout ES cells provided by the KOMP Repository and is a member of the International Mouse Phenotyping Consortium (IMPC). Associated primary phenotypic information may be found at www.mousephenotype.org. When details of the alleles have been published, the RECIPIENT shall include the following references in publications:
Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling M-C, Bottomley J, Brown SD, Burger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, et al. (2012) The mammalian gene function resource: the international knockout mouse consortium. Mamm. Genome, 23(9-10), 580-586. Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A & Skarnes WC (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature methods, 6(7), 493-495.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data