Strain Data Sheet

RBRC05564

Information1

Image
BRC No.RBRC05564
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Chx10-cre)G5Tfur
Former Common name129-BAC Chx10-Cre, 129-Chx10Cre Tg#G5
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2010. The BAC-Chx10-Cre transgene was injected into the pronuclei of B6C3F1 fertilized eggs. Mixed background (129, C57BL/6 and C3H mixed).
Strain descriptionBAC-Chx10-Cre transgenic mice. These transgenic mice express cre recombinase under the control of the mouse Chx10 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]
ReferencesJ. Neurosci., 31, 16792-16807 (2011). 22090505

Health Report

Examination Date / Room / Rack2023/03/13Room:3-2Rack:D
2022/12/12Room:3-2Rack:D
2022/09/12Room:3-2Rack:D
2022/06/13Room:3-2Rack:D
2022/03/14Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre<Tg(Chx10-cre)G5Tfur>transgene insertion 1, Takahisa Furukawamouse Vsx2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNAMouse Vsx2 genomic DNA(BAC clone, RP23-303M21), P1 phage Cre recombinase, SV40 polyA signal
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31, 16792-16807 (2011).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ikegami K, Shigeyoshi Y, Masubuchi S.
Circadian Regulation of IOP Rhythm by Dual Pathways of Glucocorticoids and the Sympathetic Nervous System.
Invest Ophthalmol Vis Sci 61(3) 26(2020) 32182332