RBRC05555
Information1 | |
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| Image | |
| BRC No. | RBRC05555 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;B6129-Ezh2<tm1Yugo>/Hko |
| Former Common name | B6-Ezh2 floxed |
| H-2 Haplotype | |
| ES Cell line | M1 [(C57BL/6 x 129)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Haruhiko Koseki and Jun Shinga, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. |
| Strain description | Ezh2 floxed mice. A floxed neo cassette was inserted upstream of exon 18 and an additional loxP was inserted downstream of exon 19 of Ezh2 gene. Conditional Ezh2 deficient mice can be generated by crossing with tissue-specific Cre mice. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Blood, 118, 6553-6561 (2011). 22042701 Neuron, 63, 600-613 (2009). 19755104 |
Health Report | |
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| Examination Date / Room / Rack | 2013/09/09Room:2-検Rack:E 2013/07/08Room:2-検Rack:E 2013/05/13Room:2-検Rack:E 2013/03/04Room:2-検Rack:E 2013/01/07Room:2-検Rack:E |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | phage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli Neomycin resistance gene, mouse Ezh2 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Haruhiko Koseki (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |