RBRC05554

Information1

Image
BRC No.RBRC05554
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Egfl6<tm1Ksek>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2012. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6J.
Strain descriptionMAEG (Egfl6) RDG motif (Arg-Gly-Asp knockout mice. Exon 9 and 10 encoding RDG motif of the Egfl6 gene were replaced with PGK-neomycin resistant gene. X linked.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]
ReferencesExp. Cell Res., 303, 148-159 (2005). 15572035
J. Cell Biol., 197(5):677-689 (2012). 22613833

Health Report

Examination Date / Room / Rack2013/05/13Room:2-検Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Egfl6EGF-like-domain, multiple 6XEgfl6<tm1Ksek>targeted mutation 1, Kiyotoshi Sekiguchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKiyotoshi Sekiguchi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data