Strain Data Sheet


Strain Information

BRC No.RBRC05552
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6.129S(Cg)-Grm6<tm1Nak>
Former Common name129-mGluR6 KO, 129-Grm6 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain129S6/SvEvTac
Strain developmentDeveloped by Shigetada Nakanishi and Takahisa Furukawa, Osaka Bioscience Institute in 2012. The mutant mice were generated by backcrossing to 129S6/SvEvTac from B6-Grm6-lacZ Tg/Grm6 KO mice (RBRC01353).
Strain descriptionmGluR6 (Grm6) knockout mice. 126 background. 1.2 kb sequence encoding part of the transmembrane region of Grm6 gene was replaced with a neomycin resistance cassette.
Colony maintenanceHeterozygote x Homozygote [or Crossing to 129S6/SvEvTac]
ReferencesJ. Neurosci., 19, 2568-2579 (1999). 10087070
Cell, 80, 757-765 (1995). 7889569

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grm6glutamate receptor, metabotropic 611Grm6<tm1Nak>targeted mutation 1, Shigetada Nakanishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAE. coli Neomycin resistance gene, Herpes simplex virus thymidine kinase promoter (HSV tk promoter), Mouse Grm6 genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 80, 757-765 (1995). J. Neurosci., 19, 2568-2579 (1999).
DepositorDrs. Shigetada Nakanishi and Takahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux MJ, Condomitti G, de Wit J, Kanagawa M, Furukawa T, Martemyanov KA.
Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors.
Cell Rep 25(1) 130-145.e5(2018) 30282023