RBRC05552

Information1

Image
BRC No.RBRC05552
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6.129S(Cg)-Grm6<tm1Nak>
Former Common name129-mGluR6 KO, 129-Grm6 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain129S6/SvEvTac
Appearance
Strain developmentDeveloped by Shigetada Nakanishi and Takahisa Furukawa, Osaka Bioscience Institute in 2012. The mutant mice were generated by backcrossing to 129S6/SvEvTac from B6-Grm6-lacZ Tg/Grm6 KO mice (RBRC01353).
Strain descriptionmGluR6 (Grm6) knockout mice. 126 background. 1.2 kb sequence encoding part of the transmembrane region of Grm6 gene was replaced with a neomycin resistance cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to 129S6/SvEvTac]
ReferencesCell, 80, 757-765 (1995). 7889569
J. Neurosci., 19, 2568-2579 (1999). 10087070

Health Report

Examination Date / Room / Rack2020/03/30Room:4-CRack:L
2020/01/27Room:4-CRack:L
2019/11/25Room:4-CRack:L
2019/09/30Room:4-CRack:L
2019/07/29Room:4-CRack:L
2019/05/27Room:4-CRack:L
2019/03/25Room:4-CRack:L
2019/01/28Room:4-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Grm6glutamate receptor, metabotropic 611Grm6<tm1Nak>targeted mutation 1, Shigetada Nakanishi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAE. coli Neomycin resistance gene, Herpes simplex virus thymidine kinase promoter (HSV tk promoter), Mouse Grm6 genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 80, 757-765 (1995). J. Neurosci., 19, 2568-2579 (1999).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorDrs. Shigetada Nakanishi and Takahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux MJ, Condomitti G, de Wit J, Kanagawa M, Furukawa T, Martemyanov KA.
Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors.
Cell Rep 25(1) 130-145(2018) 30282023