RBRC05551

Information1

Image
BRC No.RBRC05551
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf>
Former Common nameAicda-cre/rosa-tdRFP, B6-AicdaCre/ROSA26-tdRFP
H-2 Haplotype
ES Cell lineAK7 [129S4/SvJaeSor]
Background strain
Appearance
Strain developmentDeveloped by Hitoshi Nagaoka, Graduate School of Medicine, Kyoto University. Rosa-tdRFP : Hans Jorg Fehling, Institute of Immunology, University Clinics Ulm. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain descriptionThis strain has AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 tdRFP reporter (Gt(ROSA)26Sor<tm1Hjf>) alleles.
Colony maintenanceTg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
ReferencesPLoS One, 6, e29141 (2011). 22216188
Immunity, 28, 751-762 (2008). 18538592
Eur. J. Immunol., 37, 43-53 (2007). 17171761

Health Report

Examination Date / Room / Rack2016/09/12Room:2-3Rack:E
2016/07/11Room:2-3Rack:E
2016/05/16Room:2-3Rack:E
2016/03/07Room:2-3Rack:E
2016/01/12Room:2-3Rack:E
2015/11/09Room:2-3Rack:E
2015/09/28Room:2-3Rack:E
2015/09/07Room:2-3Rack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1Hjf>targeted mutation 1, Hans Jorg Fehling

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
RFPRed Fluorescent Protein (Discosoma sp.)6RFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
CD2 (human)CD2 (human)UNCD2 (human)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcremouse Aicda promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 nuclear localization sequenceUNnls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyadenylation site, yeast FRT (flipase recombination target) site, adenovirus splice acceptor site (SA), P1 phage loxP sites, E. coli neo, bovine growth hormone gene polyadenylation site, Discosoma sp. tdRFP cDNA, SV40 polyadenylation site, mouse ROSA26 locus genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Hjf. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Hans Joerg Fehling, Institute of Immunology, University Clinics Ulm to use Gt(ROSA)26Sortm1Hjf.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data