Strain Data Sheet

RBRC05541

Information1

Image
BRC No.RBRC05541
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Zfp277<tm1>
Former Common nameZfp277 KO, B6-Zfp277 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University in 2005. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionZfp277 gene knockout mice. Exon 5 of Zfp277 gene was replaced with a floxed neo cassette. Homozygous mutant mice were born according to Menderian’s law, but the growth retardation and death of homozygous mice were sporadically observed.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesPLoS One, 5, e12373 (2010). 20808772

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zfp277zinc finger protein 27712Zfp277 <tm1>targeted mutation 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNABacteriophage loxP sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Zfp277 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PloS One., 5, e12373 (2010).
DepositorAtsushi Iwama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data