RBRC05540

Information1

Image
BRC No.RBRC05540
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Brd1<tm1Hko>
Former Common nameBrd1 KO, B6-Brd1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2005. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionBrd1 gene knockout mice. Exon 2 of Brd1 gene was replaced with a floxed neo cassette. Homozygous mutant mice show abnormal embryonic development including erythropoiesis (anemia), growth retardation, open neural tube and abnormal lens morphology, and die between E12.5 and E14.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesBlood, 118, 2443-2453 (2011). 21753189

Health Report

Examination Date / Room / Rack2013/03/04Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Brd1bromodomain containing 115Brd1<tm1Hko>targeted mutation 1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), SV40 PolyA signal, Bacteriophage loxP
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 118, 2443-2453 (2011).
DepositorAtsushi Iwama (Chiba University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data