Strain Data Sheet
RBRC05539
Information1 | |
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| Image | |
| BRC No. | RBRC05539 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)> |
| Former Common name | R26Stop<FL>Bmi1, B6-ROSA26/Stop-Bmi1,GFP KI |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2009. The mutant mice were backcrossed to C57BL/6N. |
| Strain description | A loxP-flanked neo<r>-stop cassette followed by FLAG-tagged Bmil, and frt-flanked IRES-EGFP cassette, and a bovine polyA sequences were knocked in the ROSA26 locus. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | PLoS One. 7(5):e36209 (2012). 22606246 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Bmi1Bmi1 polycomb ring finger oncogene6Bmi1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | Bacteriophage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, STOP, FLAG epitope tag, mouse Bmi1 cDNA, yeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Bovine PolyA signal, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) |
| Depositor | Atsushi Iwama (Chiba University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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