Strain Data Sheet

RBRC05539

Information1

Image
BRC No.RBRC05539
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>
Former Common nameR26Stop<FL>Bmi1, B6-ROSA26/Stop-Bmi1,GFP KI
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2009. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionA loxP-flanked neo<r>-stop cassette followed by FLAG-tagged Bmil, and frt-flanked IRES-EGFP cassette, and a bovine polyA sequences were knocked in the ROSA26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesPLoS One. 7(5):e36209 (2012). 22606246

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bmi1Bmi1 polycomb ring finger oncogene6Bmi1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNABacteriophage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, STOP, FLAG epitope tag, mouse Bmi1 cDNA, yeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Bovine PolyA signal, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 未定(後日指定/will be announced)
DepositorAtsushi Iwama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data