RBRC05525

Information1

Image
BRC No.RBRC05525
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak
Former Common namePrx1-Sox9 Tg, Prx1-Sox9 transgenic, B6-Prx1-Sox9/lacZ Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The transgene was injected into the pronuclei of (C57BL/6 x DBA/2)F1 fertilized eggs. The mutant mice were crossed to C57BL/6.
Strain descriptionPrx1-Sox9/lacZ transgenic mice in which Sox9 is specifically expressed in limb bud mesenchyme. Prx1-Sox9/lacZ transgene contains a 2.4 kb of the Prx1 limb specific promoter encoding the 3xHA-epitope tagged Sox9 cDNA followe by an IRES lacZ. Tg mice exhibit pre- and postaxial extra-digit formation. lacZ expression was detected in the limb buds from E9.5
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
ReferencesMatrix Biol., 26, 224-233 (2007). 17222543

Health Report

Examination Date / Room / Rack2014/10/20Room:3-CRack:H
2014/08/18Room:3-CRack:H
2014/06/16Room:3-CRack:H
2014/04/21Room:3-CRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SOX9SRY-box containing gene 9 (human)UNSOX9mouse Prx1 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Information2

Donor DNAmouse Prx1 promoter, human influenza virus HA (hemagglutinin) tag sequence, human SOX9 cDNA, EMCV internal ribosomal entry site (ires), E. coli lacZ, bovine polyA signal
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Matrix Biol., 26, 224-233 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
DepositorBenoit de Crombrugghe (The University of Texas M. D. Anderson Cancer Center)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data