Strain Data Sheet

RBRC05517

Information1

Image
BRC No.RBRC05517
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Xbp1<tm1Tiw>
Former Common nameXBP1 KO, B6-Xbp1 KO
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times.
Strain descriptionXbp1 gene knockout mice. Exons 2-4 were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xbp1X-box binding protein 111Xbp1<tm1Tiw>targeted mutation 1, Takao Iwawaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 Phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Xbp1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data