RBRC05516
Information1 | |
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| Image | |
| BRC No. | RBRC05516 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Ern1<tm1Tiw> |
| Former Common name | IRE1alpha KO, B6-Ern1 KO |
| H-2 Haplotype | |
| ES Cell line | RF8 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times. |
| Strain description | IRE1alpha (Ern1) gene knockout mice. Exon 11-17 of Ern1 gene were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353 |
Health Report | |
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| Examination Date / Room / Rack | No Data |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter Ern1endoplasmic reticulum (ER) to nucleus signaling 111Ern1<tm1Tiw>targeted mutation 1, Takao Iwawaki Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter neoneomycin resistance gene (E. coli)11 |
Information2 | |
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| Donor DNA | バクテリオファージP1由来, loxPエレメントおよびE.coli由来, ネオマイシン耐性遺伝子 (IRE1alpha遺伝子座を破壊) |
| Research application | |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). |
| Depositor | Takao Iwawaki (Gunma University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Lab HP |
BRC mice in Publications |
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| No Data |