Strain Data Sheet

RBRC05516

Strain Information

Image
BRC No.RBRC05516
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ern1<tm1Tiw>
Former Common nameIRE1alpha KO, B6-Ern1 KO
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times.
Strain descriptionIRE1alpha (Ern1) gene knockout mice. Exon 11-17 of Ern1 gene were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability.
Iwawaki T, Akai R, Yamanaka S, Kohno K
Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ern1
MGI:1930134		endoplasmic reticulum to nucleus signalling 111Ern1<tm1Tiw>
MGI:4365396		targeted mutation 1, Takao Iwawaki
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal extraembryonic tissue physiology(MP:0004264)

  • abnormal placenta labyrinth morphology(MP:0001716)

  • abnormal placental labyrinth vasculature morphology(MP:0008803)

  • abnormal placental transport(MP:0004265)

  • abnormal trophoblast layer morphology(MP:0005031)
    • more 3 phenotypes
  • decreased embryo size(MP:0001698)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • small placenta(MP:0004259)
    • Detailed phenotype data

    Ordering Information

    Donor DNAバクテリオファージP1由来, loxPエレメントおよびE.coli由来, ネオマイシン耐性遺伝子 (IRE1alpha遺伝子座を破壊)
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    No Data