Strain Data Sheet

RBRC05516

Information1

Image
BRC No.RBRC05516
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ern1<tm1Tiw>
Former Common nameIRE1alpha KO, B6-Ern1 KO
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times.
Strain descriptionIRE1alpha (Ern1) gene knockout mice. Exon 11-17 of Ern1 gene were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ern1endoplasmic reticulum (ER) to nucleus signaling 111Ern1<tm1Tiw>targeted mutation 1, Takao Iwawaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;

Information2

Donor DNAバクテリオファージP1由来, loxPエレメントおよびE.coli由来, ネオマイシン耐性遺伝子 (IRE1alpha遺伝子座を破壊)
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data