Strain Information | |
---|---|
Image | |
BRC No. | RBRC05516 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129S4-Ern1<tm1Tiw> |
Former Common name | IRE1alpha KO, B6-Ern1 KO |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times. |
Strain description | IRE1alpha (Ern1) gene knockout mice. Exon 11-17 of Ern1 gene were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability. Iwawaki T, Akai R, Yamanaka S, Kohno K Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Ern1 MGI:1930134 | endoplasmic reticulum to nucleus signalling 1 | 11 | Ern1<tm1Tiw> MGI:4365396 | targeted mutation 1, Takao Iwawaki | |||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 11 |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | more 3 phenotypes |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | バクテリオファージP1由来, loxPエレメントおよびE.coli由来, ネオマイシン耐性遺伝子 (IRE1alpha遺伝子座を破壊) |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). |
Depositor | Takao Iwawaki (Gunma University) |
Strain Status | ![]() |
Strain Availability | ![]() |
Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |