Strain Data Sheet

RBRC05515

Information1

Image
BRC No.RBRC05515
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ern1<tm2.1Tiw>
Former Common nameIRE1alpha floxed, B6-Ern1/dNeo floxed
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. Frt flanked neo cassette was removed by crossing with FLP mice. C57BL/6 and 129 mixed background.
Strain descriptionIRE1alpha (Ern1) gene floxed mice. Exons 20 and 21 were flanked by loxP sites. Conditional IRE1alpha deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack2022/08/22Room:4-ARack:I
2022/05/23Room:4-ARack:I
2022/03/07Room:4-ARack:I
2021/12/06Room:4-ARack:I
2021/09/08Room:4-ARack:I
2021/06/07Room:4-ARack:I
2021/03/22Room:4-ARack:I
2021/01/29Room:4-ARack:I
2020/12/14Room:4-ARack:I
2020/10/05Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ern1endoplasmic reticulum (ER) to nucleus signaling 111Ern1<tm2.1Tiw>targeted mutation 2.1, Takao Iwawaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ern1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data