Strain Data Sheet

RBRC05515

Strain Information

Image
BRC No.RBRC05515
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ern1<tm2.1Tiw>
Former Common nameIRE1alpha floxed, B6-Ern1/dNeo floxed
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. Frt flanked neo cassette was removed by crossing with FLP mice. C57BL/6 and 129 mixed background.
Strain descriptionIRE1alpha (Ern1) gene floxed mice. Exons 20 and 21 were flanked by loxP sites. Conditional IRE1alpha deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability.
Iwawaki T, Akai R, Yamanaka S, Kohno K
Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack2025/07/28Room:4-ARack:ISentinel mouse program
2025/05/19Room:4-ARack:ISentinel mouse program
2025/02/17Room:4-ARack:ISentinel mouse program
2024/11/18Room:4-ARack:ISentinel mouse program
2024/08/19Room:4-ARack:ISentinel mouse program
2024/05/20Room:4-ARack:ISentinel mouse program
2024/02/26Room:4-ARack:IAdditional tests
2024/02/19Room:4-ARack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ern1
MGI:1930134		endoplasmic reticulum to nucleus signalling 111Ern1<tm2.1Tiw>
MGI:4365397		targeted mutation 2.1, Takao Iwawaki
Frt yeast FRT (flippase recombination target) site11Frt
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ern1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Li C, Tan YP, Gao D, Su R, Xu K, Liu SC, Li XF, Lu YH, Yi LT, Wang G, Ou XH, Meng TG, Sun QY.
    IRE1α RNase activity is critical for early embryo development by degrading maternal transcripts.
    Nucleic Acids Res 53(11) (2025) 40530690
    McCright SJ, Harding O, Chini J, DeMarco N, Hung LY, Pastore CF, Lu W, Rabinowitz J, Henao-Mejia J, Herbert DR, Young LR, Hill DA.
    Dietary saturated fatty acids promote lung myeloid cell inflammasome activation and IL-1β-mediated inflammation in mice and humans.
    Sci Transl Med 17(813) eadp5653(2025) 40864681