RBRC05508
Information1 | |
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| Image | |
| BRC No. | RBRC05508 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Dnajc10<tm1Tiw> |
| Former Common name | ERdj5 KO, B6-Dnajc10 |
| H-2 Haplotype | |
| ES Cell line | RF8 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2007. The mutant mice were crossed to C57BL/6J. |
| Strain description | ERdj5 (Dnajc10) gene knockout mice. Exon 3 of Dnajc10 gene was replaced with a floxed neo cassette. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Biochem. J., 425, 117-125 (2009). 19788412 |
Health Report | |
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| Examination Date / Room / Rack | 2015/07/27Room:4-ARack:J 2015/05/25Room:4-ARack:J 2015/04/08Room:4-ARack:J 2015/04/06Room:4-ARack:J 2015/03/23Room:4-ARack:J 2015/01/19Room:4-ARack:J 2014/11/17Room:4-ARack:J 2014/09/16Room:4-ARack:J |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter Dnajc10DnaJ (Hsp40) homolog, subfamily C, member 102Dnajc10<tm1Tiw>targeted mutation 1, Takao Iwawaki Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele namePromoter neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | Phage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Dnajc10 genomic DNA |
| Research application | |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. J., 425, 117-125 (2010). |
| Depositor | Takao Iwawaki (Gunma University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Lab HP |
BRC mice in Publications |
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| No Data |