Strain Data Sheet

RBRC05508

Information1

Image
BRC No.RBRC05508
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnajc10<tm1Tiw>
Former Common nameERdj5 KO, B6-Dnajc10
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2007. The mutant mice were crossed to C57BL/6J.
Strain descriptionERdj5 (Dnajc10) gene knockout mice. Exon 3 of Dnajc10 gene was replaced with a floxed neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. J., 425, 117-125 (2009). 19788412

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnajc10DnaJ (Hsp40) homolog, subfamily C, member 102Dnajc10<tm1Tiw>targeted mutation 1, Takao Iwawaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Dnajc10 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. J., 425, 117-125 (2010).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data