Strain Data Sheet

RBRC05508

Strain Information

Image
BRC No.RBRC05508
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnajc10<tm1Tiw>
Former Common nameERdj5 KO, B6-Dnajc10
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2007. The mutant mice were crossed to C57BL/6J.
Strain descriptionERdj5 (Dnajc10) gene knockout mice. Exon 3 of Dnajc10 gene was replaced with a floxed neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Positive contribution of ERdj5/JPDI to endoplasmic reticulum protein quality control in the salivary gland.
Hosoda A, Tokuda M, Akai R, Kohno K, Iwawaki T
Biochem. J., 425, 117-125 (2009). 19788412

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnajc10DnaJ heat shock protein family (Hsp40) member C102Dnajc10targeted mutation 1, Takao Iwawaki
loxPphage P1 loxP2loxP
loxPphage P1 loxP2loxP
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal salivary gland physiology(MP:0005310)

  • endocrine/exocrine gland phenotype(MP:0005379)

  • homeostasis/metabolism phenotype(MP:0005376)

  • increased endoplasmic reticulum stress(MP:0020354)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Dnajc10 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. J., 425, 117-125 (2010).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Jeong H, Hong EH, Ahn JH, Cho J, Jeong JH, Kim CW, Yoon BI, Koo JH, Park YY, Yang YM, Iwawaki T, Vallance BA, Chang SY, Ko HJ.
    ERdj5 protects goblet cells from endoplasmic reticulum stress-mediated apoptosis under inflammatory conditions.
    Exp Mol Med (2023) 36759578
    Kim MS, Yi EJ, Kim YI, Kim SH, Jung YS, Kim SR, Iwawaki T, Ko HJ, Chang SY.
    ERdj5 in Innate Immune Cells Is a Crucial Factor for the Mucosal Adjuvanticity of Cholera Toxin.
    Front Immunol 10 1249(2019) 31275300