RBRC05502

Information1

Image
BRC No.RBRC05502
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Cfm2<tm1Tfur>
Former Common name129-Cfm2 KO
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2007. TC1/TC-1 ES cells were used. 129 and C57BL/6 mixed background.
Strain descriptionCfm2 gene knockout mice. Exon 1 of Cfm2 gene was replaced with PGK-neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote, Heterozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack2013/12/16Room:3-BRack:F
2013/10/21Room:3-BRack:F
2013/08/26Room:3-BRack:F
2013/06/17Room:3-BRack:F
2013/04/15Room:3-BRack:F
2012/12/10Room:3-BRack:F
2012/10/15Room:3-BRack:F
2012/08/27Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fam101afamily with sequence similarity 101, member A5Fam101a <tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAMouse PGK-neo promoter, SV40 Poly A signal, mouse Fam101a genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 未定(後日指定)Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data