RBRC05501

Information1

Image
BRC No.RBRC05501
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Tmem119<tm1Tfur>
Former Common name129-Obif KO, 129-Obif<tm1Tfur>
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background.
Strain descriptionObif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette. Homozygous mutant mice show lower bone mass than controls at 14 days and 8 weeks old.
Colony maintenanceHomozygote x Heterozygote [or Crossing to 129S6/SvEvTac]
ReferencesBMC Dev. Biol., 9, 70 (2009). 20025746
Dev. Growth Differ., 54(4):474-480 (2012). 22416756

Health Report

Examination Date / Room / Rack2018/10/15Room:3-BRack:G
2018/08/20Room:3-BRack:G
2018/06/18Room:3-BRack:G
2018/04/16Room:3-BRack:G
2018/02/19Room:3-BRack:G
2017/12/18Room:3-BRack:G
2017/10/16Room:3-BRack:G
2017/08/21Room:3-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tmem119transmembrane protein 1195Tmem119 <tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 Poly A signal, mouse Tmem119 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth Differ., 54(4):474-480 (2012).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data