Strain Data Sheet

RBRC05496

Strain Information

Image
BRC No.RBRC05496
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Alk<tm1(hALK*R1275Q)Homy>
Former Common nameALK KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionHuman ALK (R1275Q variant) gene knock-in mice. hALK (R1275Q) cDNA-IRES-beta geo-polyA was inserted into the middle of the exon 21 and exon 22.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ALK anaplastic lymphoma kinase (human)17ALK
Alk
MGI:103305		anaplastic lymphoma kinase17Alk <tm1(hALK*R1275Q)Homy> targeted mutation 1, Hiroaki Honda
  • neuroblastoma, susceptibility to, 3(MedGEN)
    • IRES internal ribosomal entry site (EMCV)17
    lacZ beta-galactosidase (E. coli)17
    loxP phage P1 loxP17loxP
    poly A polyA17poly A

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • increased neuroblastoma incidence (MP:0002039)

  • increased tumor incidence (MP:0002020)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse ALK genomic DNA, human ALK cDNA, Phage P1 LoxP, E. coli beta-galactosidase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), polyA sequence (synthetic DNA)
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data