Strain Data Sheet

RBRC05496

Strain Information

Image
BRC No.RBRC05496
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Alk<tm1(hALK*R1275Q)Homy>
Former Common nameALK KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionHuman ALK (R1275Q variant) gene knock-in mice. hALK (R1275Q) cDNA-IRES-beta geo-polyA was inserted into the middle of the exon 21 and exon 22.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Alkanaplastic lymphoma kinase17Alk <tm1(hALK*R1275Q)Homy>targeted mutation 1, Hiroaki Honda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ALKanaplastic lymphoma kinase (human)17ALK

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)17internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ApolyA17poly A

Ordering Information

Donor DNAmouse ALK genomic DNA, human ALK cDNA, Phage P1 LoxP, E. coli beta-galactosidase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), polyA sequence (synthetic DNA)
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data