Strain Data Sheet

RBRC05495

Strain Information

Image
BRC No.RBRC05495
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Tnfaip3<tm1.1Homy>
Former Common nameA20 KO, B6-Tnfaip3 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionA20 (Tnfaip3) gene knockout mice. Floxed Exon 3 of A20 gene was deleted by crossing Cre delete mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.
Akiko Nagamachi, Yuichiro Nakata, Takeshi Ueda, Norimasa Yamasaki, Yasuhiro Ebihara, Kohichiro Tsuji, Zen-ichiro Honda, Keiyo Takubo, Toshio Suda, Hideaki Oda, Toshiya Inaba, Hiroaki Honda
PLoS One. 9(1):e87425 (2014). 24498102

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Frt yeast FRT (flippase recombination target) site10Frt
Tnfaip3
MGI:1196377		tumor necrosis factor, alpha-induced protein 310Tnfaip3
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal B cell number (MP:0002458)

  • abnormal blood cell morphology/development (MP:0002429)

  • abnormal circulating cytokine level (MP:0010210)

  • abnormal circulating interleukin level (MP:0008588)

  • abnormal corpus callosum morphology (MP:0000780)
    • more 30 phenotypes
  • abnormal liver morphology (MP:0000598)

  • abnormal macrophage cell number (MP:0020202)

  • abnormal myelopoiesis (MP:0001601)

  • abnormal spleen morphology (MP:0000689)

  • abnormal survival (MP:0010769)

  • anemia (MP:0001577)

  • cachexia (MP:0005150)

  • decreased B cell number (MP:0005017)

  • decreased body weight (MP:0001262)

  • decreased dendritic cell number (MP:0008127)

  • decreased monocyte cell number (MP:0000223)

  • enlarged liver (MP:0000599)

  • enlarged lymph nodes (MP:0000702)

  • hematoma (MP:0008817)

  • increased B cell number (MP:0005014)

  • increased apoptosis (MP:0006042)

  • increased circulating interferon-gamma level (MP:0008577)

  • increased circulating interleukin-1 beta level (MP:0008641)

  • increased circulating interleukin-6 level (MP:0008596)

  • increased circulating tumor necrosis factor level (MP:0008553)

  • increased dendritic cell number (MP:0008126)

  • increased neuron apoptosis (MP:0003203)

  • increased single-positive T cell number (MP:0008082)

  • increased susceptibility to xenobiotic induced morbidity/mortality (MP:0009766)

  • liver inflammation (MP:0001860)

  • lung inflammation (MP:0001861)

  • pale spleen (MP:0009246)

  • premature death (MP:0002083)

  • senility (MP:0002571)

  • thymus atrophy (MP:0003644)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfaip3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month May 2014

    BRC mice in Publications

    No Data