Strain Information | |
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Image | |
BRC No. | RBRC05495 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Tnfaip3<tm1.1Homy> |
Former Common name | A20 KO, B6-Tnfaip3 KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice. |
Strain description | A20 (Tnfaip3) gene knockout mice. Floxed Exon 3 of A20 gene was deleted by crossing Cre delete mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References | Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function. Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H PLoS One. 9(1):e87425 (2014). 24498102 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 10 | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | 10 | Frt | ||||
Tnfaip3 | tumor necrosis factor, alpha-induced protein 3 | 10 | Tnfaip3 | ||||
loxP | phage P1 loxP | 10 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 10 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 31 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, yeast FRT (flipase recombination target) sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfaip3 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2014 |
BRC mice in Publications |
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No Data |