RBRC05495

Information1

Image
BRC No.RBRC05495
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Tnfaip3<tm1.1Homy>
Former Common nameA20 KO, B6-Tnfaip3 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionA20 (Tnfaip3) gene knockout mice. Floxed Exon 3 of A20 gene was deleted by crossing Cre delete mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesPLoS One. 9(1):e87425 (2014). 24498102

Health Report

Examination Date / Room / Rack2014/07/07Room:2-3Rack:C
2014/05/12Room:2-3Rack:C
2014/03/10Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tnfaip3tumor necrosis factor, alpha-induced protein 310Tnfaip3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfaip3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month May 2014

BRC mice in Publications

No Data