Strain Data Sheet


Strain Information

BRC No.RBRC05494
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Tnfaip3<tm1Homy>
Former Common nameA20 cKO, B6-Tnfaip3 floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionA20 (Tnfaip3) gene floxed mice. Exon 3 of A20 gene was flanked by loxP sites. Conditional A20 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesPLoS One, 9(1):e87425 (2014). 24498102

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnfaip3tumor necrosis factor, alpha-induced protein 310Tnfaip3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Tnfaip3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month May 2014

BRC mice in Publications

Yusuke Yokoyama, Tomohiro Tamachi, Arifumi Iwata, Yuko Maezawa, Kazuyuki Meguro, Masaya Yokota, Hiroaki Takatori, Akira Suto, Kotaro Suzuki, Koichi Hirose, Noritaka Yamaguchi, Hiroaki Honda, Hiroshi Nakajima
A20 (Tnfaip3) expressed in CD4+ T cells suppresses Th2 cell-mediated allergic airway inflammation in mice
Biochemical and Biophysical Research Communications 629 47-53(2022) 36099784

Liu SS, Lv XX, Liu C, Qi J, Li YX, Wei XP, Li K, Hua F, Cui B, Zhang XW, Yu JJ, Yu JM, Wang F, Shang S, Zhao CX, Hou XY, Yao ZG, Li PP, Li X, Huang B, Hu ZW.
Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages.
Immunity 51(3) 522-534.e7(2019) 31471107

Montarolo F, Perga S, Tessarolo C, Spadaro M, Martire S, Bertolotto A.
TNFAIP3 Deficiency Affects Monocytes, Monocytes-Derived Cells and Microglia in Mice.
Int J Mol Sci 21(8) (2020) 32325694