Strain Data Sheet

RBRC05494

Strain Information

Image
BRC No.RBRC05494
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Tnfaip3<tm1Homy>
Former Common nameA20 cKO, B6-Tnfaip3 floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain descriptionA20 (Tnfaip3) gene floxed mice. Exon 3 of A20 gene was flanked by loxP sites. Conditional A20 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References
Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.
Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H
PLoS One, 9(1):e87425 (2014). 24498102

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Tnfaip3
MGI:1196377		tumor necrosis factor, alpha-induced protein 310Tnfaip3
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell number(MP:0002458)

  • abnormal blood cell morphology/development(MP:0002429)

  • abnormal circulating cytokine level(MP:0010210)

  • abnormal circulating interleukin level(MP:0008588)

  • abnormal corpus callosum morphology(MP:0000780)
    • more 31 phenotypes
  • abnormal liver morphology(MP:0000598)

  • abnormal macrophage cell number(MP:0020202)

  • abnormal myelopoiesis(MP:0001601)

  • abnormal spleen morphology(MP:0000689)

  • abnormal survival(MP:0010769)

  • anemia(MP:0001577)

  • cachexia(MP:0005150)

  • decreased B cell number(MP:0005017)

  • decreased body weight(MP:0001262)

  • decreased dendritic cell number(MP:0008127)

  • decreased monocyte cell number(MP:0000223)

  • enlarged liver(MP:0000599)

  • enlarged lymph nodes(MP:0000702)

  • hematoma(MP:0008817)

  • hematopoietic system phenotype(MP:0005397)

  • increased B cell number(MP:0005014)

  • increased apoptosis(MP:0006042)

  • increased circulating interferon-gamma level(MP:0008577)

  • increased circulating interleukin-1 beta level(MP:0008641)

  • increased circulating interleukin-6 level(MP:0008596)

  • increased circulating tumor necrosis factor level(MP:0008553)

  • increased dendritic cell number(MP:0008126)

  • increased neuron apoptosis(MP:0003203)

  • increased single-positive T cell number(MP:0008082)

  • increased susceptibility to xenobiotic induced morbidity/mortality(MP:0009766)

  • liver inflammation(MP:0001860)

  • lung inflammation(MP:0001861)

  • pale spleen(MP:0009246)

  • premature death(MP:0002083)

  • senility(MP:0002571)

  • thymus atrophy(MP:0003644)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Tnfaip3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month May 2014

    BRC mice in Publications

    Yusuke Yokoyama, Tomohiro Tamachi, Arifumi Iwata, Yuko Maezawa, Kazuyuki Meguro, Masaya Yokota, Hiroaki Takatori, Akira Suto, Kotaro Suzuki, Koichi Hirose, Noritaka Yamaguchi, Hiroaki Honda, Hiroshi Nakajima
    A20 (Tnfaip3) expressed in CD4+ T cells suppresses Th2 cell-mediated allergic airway inflammation in mice
    Biochemical and Biophysical Research Communications 629 47-53(2022) 36099784
    Montarolo F, Perga S, Tessarolo C, Spadaro M, Martire S, Bertolotto A.
    TNFAIP3 Deficiency Affects Monocytes, Monocytes-Derived Cells and Microglia in Mice.
    Int J Mol Sci 21(8) (2020) 32325694
    Liu SS, Lv XX, Liu C, Qi J, Li YX, Wei XP, Li K, Hua F, Cui B, Zhang XW, Yu JJ, Yu JM, Wang F, Shang S, Zhao CX, Hou XY, Yao ZG, Li PP, Li X, Huang B, Hu ZW.
    Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages.
    Immunity 51(3) 522-534.e7(2019) 31471107