RBRC05477

Information1

Image
BRC No.RBRC05477
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NDE-hsp-Cluap1,lacZ)41Hmd
Former Common nameNDE-hsp Qilin-lacZ (41), B6-NDE-Qilin/lacZ Tg#41
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. 0.7 kb segment containing Nodal NDE enhancer and Qilin cDNA-IRES LacZ genes were injected into the pronuclei of ((C57BL/6 x BALB/c)F1 x B6129-Qilin<tm1.1Hmd>)F1 fertilized eggs (2 copies). C57BL/6 and 129 mixed background.
Strain descriptionThis transgenic mouse expresses Cluap1 (Qilin) and lacZ genes in crown cells of embryonic node. Tg expression can be monitored by X-gal staining.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cluap1clusterin associated protein 1UNCluap1mouse Nodal NDE-enhancer, mouse Hsp68 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNAMouse Nodal NDE-enhancer, Mouse Hsp68 promoter, Mouse Qilin gene cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ gene, phage P1 loxP sequence
Research applicationCre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data