RBRC05474

Information1

Image
BRC No.RBRC05474
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Foxj1-Cluap1,lacZ)14Hmd
Former Common nameFxJ1-1.1 Qilin-lacZ (14), B6-Foxj1-Qilin/LacZ Tg#14
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. 1.1 kb segment containing human FoxJ1 promoter and Qilin cDNA-IRES LacZ genes were injected into the pronuclei of ((C57BL/6 x BALB/c)F1 x B6129-Qilin<tm1.1Hmd>)F1 fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionTransgene specifically express Cluap1 (Qilin) and LacZ reporter in pit cells of embryonic node. Tg expression can be monitored by X-gal staining.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cluap1clusterin associated protein 1UNCluap1human Foxj1 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Information2

Donor DNAHuman Foxj1 promoter, Mouse Qilin gene cDNA, Virus IRES sequence, E. coli LacZ gene
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data