Strain Data Sheet



BRC No.RBRC05473
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Mad2l2<tm1Myo>
Former Common nameRev7 KO, B6-Mad2l2 KO
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Strain developmentDeveloped by Yoshiki Murakumo, Nagoya University Graduate School of Medicine in 2009. The mutant mice were backcrossed to C57BL/6.
Strain descriptionRev7 (Mad2l2) knockout mice. Homozygous mutant mice lack germ cells due to apoptosis of primordial germ cell in the middle phase of the embryonic development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesJ. Biol. Chem., 275, 4391-4397 (2000). 10660610
J. Biol. Chem., 288(15):10459-10471 (2013). 23463509

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mad2l2MAD2 mitotic arrest deficient-like 2 (yeast)4Mad2l2<tm1Ymu>targeted mutation 1, Yoshiki MurakumoRev7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAMouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, Mouse Rev7 gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 288(15):10459-10471 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoshiki Murakumo (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data