RBRC05472

Information1

Image
BRC No.RBRC05472
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>
Former Common nameBALB-ROSA26/CAG-tdTomato
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain descriptionThe mice having a CAG-tdTomato-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
ReferencesNucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack2020/06/23Room:3-BRack:C
2020/06/01Room:3-BRack:C
2020/02/17Room:3-BRack:C
2019/12/16Room:3-BRack:C
2019/10/21Room:3-BRack:C
2019/08/26Room:3-BRack:C
2019/06/17Room:3-BRack:C
2019/04/15Room:3-BRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>targeted mutation 6.1, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tdTomatoRed Fluorescent Protein (Discosoma sp.) tdTomato (tandem dimeric Tomato)6tdTomatoCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Information2

Donor DNAadenovirus major late transcript splice acceptor sequence (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. tdTomato cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 lox2272 site
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorMasato Ohtsuka (Tokai University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.UCSD MTA
Genotyping protocol -PCR-

BRC mice in Publications

No Data