Strain Data Sheet

RBRC05468

Information1

Image
BRC No.RBRC05468
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm5.1(CAG-mOrange)Maoh>
Former Common nameB6-ROSA26/CAG-mOrange
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain descriptionThe mice having a CAG-mOrange-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm5.1(CAG-mOrange)Maoh>targeted mutation 5.1, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mOrangeOrange Fluorescent Protein (Discosoma sp.)6mOrangeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Information2

Donor DNACAGプロモーター, mOrange, SV40 polyA, FRT, lox2272
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasato　Ohtsuka (Tokai University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data