RBRC05466

Information1

Image
BRC No.RBRC05466
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>
Former Common nameB6-ROSA26/CAG-GFP
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLPe injection.
Strain descriptionThe mice having a CAG-EGFP-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:A
2020/02/17Room:3-ARack:A
2019/12/16Room:3-ARack:A
2019/10/21Room:3-ARack:A
2019/08/26Room:3-ARack:A
2019/04/15Room:3-ARack:G
2019/02/18Room:3-ARack:G
2018/12/17Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>targeted mutation 2.1, Masato Ohtsuka

Information2

Donor DNAyeast FRT (flipase recombination target) site, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish EGFP cDNA, SV40 polyA, Phage P1 lox2272 site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasato Ohtsuka (Tokai University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data