RBRC05457

Information1

Image
BRC No.RBRC05457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Nedd9<tm1Miku>
Former Common nameCas-L KO B6-Nedd9 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Sachiko Seo, Faculty of Medicine, University of Tokyo in 2001. The targeting vector was transferred into TT2 ES cells derived from (C57BL/6 x CBA) F1. The mutant mice were backcrossed to C57BL/6.
Strain descriptionCas-L (Nedd9, neural precursor cell expressed, developmentally down-regulated gene 9) gene knockout mice. A 1.2 kb region including exon 2 was replaced with EGFP and a floxed neomycin resistant cassette. Homozygous mutant mice show reduced numbers of lymphocytes in secondary lymphoid organs and an almost complete loss of marginal zone B (MZB) cells in the spleen.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Immunol., 175, 3492-3501 (2005). 16148091
Cancer Res., 69, 7198-7206 (2009). 19738060
Cancer Sci., 102, 2109-2117 (2011). 21848808

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nedd9neural precursor cell expressed, developmentally down-regulated gene 913Nedd9<tm1Miku>targeted mutation 1, Mineo Kurokawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)13EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, Jellyfish EGFP cDNA, mouse Cas-L (HEF1/NEDD9) genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 175, 3492-3501 (2005).
DepositorMineo Kurokawa (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data