Strain Data Sheet

RBRC05455

Information1

Image
BRC No.RBRC05455
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mtap7d1<tm1>/2A5
Former Common nameB6-Mtap7d1 KO, B6.Cg-Mtap7d1<tm1>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Atsuko Mizuno and Makoto Suzuki, Jichi Medical University, School of Medicine in 2005. Bruce 4 ES cells were used. The mutant mice were crossed to C57BL/6JJcl.
Strain descriptionMtap7d1, MAP7 domain containing 1, gene knockout mice. Homozygous mutant mice die soon after birth due to their respiratory failure.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map7d1MAP7 domain containing 14Map7d1<tm1Msz>targeted mutation 1, Makoto SuzukiMtap7d1, Parcc1, Rprc1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Map7d1 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT must obtain a prior written consent on the use of BIOLOGICAL RESOURCE from the DEVELOPER (Dr. Makoto Suzuki). The RECIPENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the the DEVELOPER.
DepositorAtsuko Mizuno (Jichi Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data