Information1 | |
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Image | |
BRC No. | RBRC05454 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk> |
Former Common name | tetO-ChR2 KI, B6-tetO-ChR2/YFP KI |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. (C57BL/6 x 129) F1 ES cells were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background. |
Strain description | The tetO-ChR2 (C128S) cassette was inserted just downstream of the polyadenylation signal of Actb gene. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Nat. Neurosci., 22(5):770-777 (2019). 30988523 Cell Rep., 36(4):109427(2021). 34320360 Cell Rep., 2(2):397-406 (2012). 22854021 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Information2 | |
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Donor DNA | Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Rep., 2(2):397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper. |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Apr 2013 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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