Strain Data Sheet

RBRC05454

Information1

Image
BRC No.RBRC05454
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk>
Former Common nametetO-ChR2 KI, B6-tetO-ChR2/YFP KI
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. (C57BL/6 x 129) F1 ES cells were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background.
Strain descriptionThe tetO-ChR2 (C128S) cassette was inserted just downstream of the polyadenylation signal of Actb gene. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesNat. Neurosci., 22(5):770-777 (2019). 30988523
Cell Rep., 36(4):109427(2021). 34320360
Cell Rep., 2(2):397-406 (2012). 22854021

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb <<tm1(tetO-ChR2*C128S/EYFP)Kftnk>>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Information2

Donor DNAPhage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Rep., 2(2):397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Apr 2013
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data