Strain Data Sheet


Strain Information

BRC No.RBRC05454
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk>
Former Common nametetO-ChR2 KI, B6-tetO-ChR2/YFP KI
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Strain developmentDeveloped by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. (C57BL/6 x 129) F1 ES cells were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background.
Strain descriptionThe tetO-ChR2 (C128S) cassette was inserted just downstream of the polyadenylation signal of Actb gene. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesCell Rep., 2(2):397-406 (2012). 22854021
Nat. Neurosci., 22(5):770-777 (2019). 30988523
Cell Rep., 36(4):109427(2021). 34320360

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb <<tm1(tetO-ChR2*C128S/EYFP)Kftnk>>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Ordering Information

Donor DNAPhage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Rep., 2(2):397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Apr 2013

BRC mice in Publications

Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka KF.
Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.
Cell Rep 36(4) 109427(2021) 34320360

Oishi M, Passlick S, Yamazaki Y, Unekawa M, Adachi R, Yamada M, Imayoshi I, Abe Y, Steinhäuser C, Tanaka KF.
Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter.
Glia 71(2) 317-333(2023) 36165697

Kato T, Mitsukura Y, Yoshida K, Mimura M, Takata N, Tanaka KF.
Oscillatory population-level activity of dorsal raphe serotonergic neurons is inscribed in sleep structure.
J Neurosci 42(38) 7244-55(2022) 35970565

Yamazaki Y, Abe Y, Fujii S, Tanaka KF.
Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain.
Nat Commun 12(1) 5146(2021) 34446732

Kono A, Shikano Y, Tanaka KF, Yamaura K, Tsutsui-Kimura I.
Inhibition of the dorsomedial striatal direct pathway is essential for the execution of action sequences.
Neuropsychopharmacol Rep 43(3) 414-424(2023) 37553985

Murata J, Unekawa M, Kudo Y, Kotani M, Kanno I, Izawa Y, Tomita Y, Tanaka KF, Nakahara J, Masamoto K.
Acceleration of the Development of Microcirculation Embolism in the Brain due to Capillary Narrowing.
Stroke 54(8) 2135-2144(2023) 37309687