RBRC05451

Information1

Image
BRC No.RBRC05451
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nlgn3<tm1(STOP-tetO)Rhn>
Former Common nameNlgn3 STOPtetO KI, B6-Nlgn3/STOPtetO KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. C57BL/6 and 129 mixed background.
Strain descriptionNlgn3 knockout mice. Neo-STOP-tetO (loxP-FRT-Neo-STOP-FRT-tetO-loxP) cassette was inserted just upstream of the Nlgn3 gene. Homozygous mutant mice show no obvious abnormality. tet0 mediated allele can be generated by crossing with FLP mice.
Colony maintenanceHomozygote x Hemizygote [or Crossing to C57BL/6JJcl]
ReferencesBiol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack2013/07/22Room:4-ARack:I
2013/07/17Room:4-ARack:I
2013/07/16Room:4-ARack:I
2013/05/20Room:4-ARack:I
2013/03/25Room:4-ARack:I
2013/01/21Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nlgn3neuroligin 3XNlgn3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)XMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tetOtetracycline operator (E. coli)XtetO

Information2

Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) sites, mouse PGK promoter, bacterial EM7 promoter (Synthetic), E. coli neo, yeast His3 3' UTR, SV40 virus SV40 polyA signal, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, mouse Nlgn3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data