Strain Data Sheet


Strain Information

BRC No.RBRC05450
SpeciesMus musculus
Strain nameB6;CBB6(129)-Tg(Mlc1-tTA)2Rhn
Former Common nameMlc1-tTA#2, B6-Mlc1-tTA Tg#2
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. C57BL/6 and 129S6 mixed background.
Strain descriptionMlc1-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse Mlc1 promoter. tTA expression is detected in Mlc1 positive astrocytes.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesBiol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse Mlc1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAmouse Mlc1 genomic DNA, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal
Research applicationTet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Chen J, He W, Hu X, Shen Y, Cao J, Wei Z, Luan Y, He L, Jiang F, Tao Y.
A role for ErbB signaling in the induction of reactive astrogliosis.
Cell Discov 3 17044(2017) 29238610

Tsunematsu T, Sakata S, Sanagi T, Tanaka KF, Matsui K.
Region-Specific and State-Dependent Astrocyte Ca2+ Dynamics during the Sleep-Wake Cycle in Mice.
J Neurosci 41(25) 5440-5452(2021) 34006590