Strain Data Sheet


Strain Information

BRC No.RBRC05447
SpeciesMus musculus
Strain nameB6.Cg-Tg(ACTB-tTS)1Mllo
Former Common nameActin-tTS Tg, B6-ACTB-tTS Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Moises Mallo, Max-Plank Institute of Immunobiology. C57BL/6 and 129 mixed background.
Strain descriptionACTB-tTS transgenic mice. These mice express tetracycline-controlled transcriptional silencer (tTS) gene under the control of the human beta actin promoter. By the combination with homozygous tetO-modified allele, expression from the gene can be blocked in the absence of doxycycline and restore when the antibiotic is administered to the animal.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesGenomics, 81, 356-360 (2003). 12676559
Nat. Commun., 12(1) 5146 (2021). 34446732
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTSE. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNAUNtTStransgene insertion 1, Moises Mallohuman beta-actin promoter, E. coli Tetracycline repressor (tetR), human Zfp KRAB domain

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAhuman beta actin promoter, tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA), chicken globin genomic DNA, SV40 polyA signal
Research applicationTet system
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEVELOPER (Dr. Moises Mallo, (Max-Plank Institute of Immunobiology) is requested. Genomics, 81(4):356-360 2003. The RECIPIENT must contact the DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEVELOPER and must obtain a prior written consent from the DEVELOPER.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data