Strain Data Sheet

RBRC05446

Strain Information

Image
BRC No.RBRC05446
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(129)-Tg(Plp1-tTA)1Ike
Former Common namePLP-tTA Tg, B6-PLP-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionPLP-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse PLP promoter. tTA expression is detected in oligodendrocytes.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain.
Yoshihiko Yamazaki, Yoshifumi Abe, Satoshi Fujii, Kenji F Tanaka
Nat. Commun., 12(1) 5146 (2021). 34446732

Gene induction in mature oligodendrocytes with a PLP-tTA mouse line.
Inamura N, Sugio S, Macklin W B, Tomita K, Tanaka K F, Ikenaka K
Genesis, 50(5):424-8 (2012). 21953949

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • hereditary spastic paraplegia 2(DisGeNET, MedGEN)

  • Pelizeaus-Merzbacher spectrum disorder(DisGeNET, MedGEN)
    • tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse PLP promoter
  • hereditary spastic paraplegia 2(DisGeNET, MedGEN)

  • Pelizeaus-Merzbacher spectrum disorder(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PLP promoter, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal
    Research applicationTet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 50(5):424-8 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamaguchi H, Nishimura Y, Matsuse D, Sekiya H, Masaki K, Tanaka T, Saiga T, Harada M, Kira YI, Dickson DW, Fujishima K, Matsuo E, Tanaka KF, Yamasaki R, Isobe N, Kira JI.
    A rapidly progressive multiple system atrophy-cerebellar variant model presenting marked glial reactions with inflammation and spreading of α-synuclein oligomers and phosphorylated α-synuclein aggregates.
    Brain Behav Immun 121 122-141(2024) 38986725