RBRC05446

Information1

Image
BRC No.RBRC05446
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(129)-Tg(Plp1-tTA)1Ike
Former Common namePLP-tTA Tg, B6-PLP-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionPLP-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse PLP promoter. tTA expression is detected in oligodendrocytes.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesGenesis, 50(5):424-8 (2012). 21953949

Health Report

Examination Date / Room / Rack2015/11/30Room:4-ARack:K
2015/09/28Room:4-ARack:K
2015/07/27Room:4-ARack:K
2015/05/25Room:4-ARack:K
2015/03/23Room:4-ARack:K
2015/01/19Room:4-ARack:K
2014/11/17Room:4-ARack:K
2014/09/16Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse PLP promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse PLP promoter, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal
Research applicationTet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. [Htr5B-tTA KI: (will be announced), PLP-tTA Tg: Genesis.2011 Sep 22. doi:10. 1002/dvg. 20808.] The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data