Strain Data Sheet

RBRC05445

Information1

Image
BRC No.RBRC05445
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Htr5b<tm1(tTA)Rhn>
Former Common nameHtr5B-tTA KI, B6-Htr5b/tTA KI
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. The mutant mice were crossed to C57BL/6J over 6 times.
Strain descriptionHtr5B-tTA knock-in mice. The tetracycline-controlled transactivator (tTA) gene was inserted into the Htr5b gene. tTA expression is detected in medial habenular and inferior olivary nuclei, and hippocampus CA1 region.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenesis, 50(5):424-8 (2012). 21953949
Hippocampus 33296119
Cell Rep., 2(2):397-406 (2012). 22854021
Nat. Neurosci., 22(5):770-777 (2019). 30988523

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Htr5b5-hydroxytryptamine (serotonin) receptor 5B1Htr5b <tm1(tTA)Rhn>targeted mutation 1, Rene Hen

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA1HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)1tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain

Information2

Donor DNAtTA(E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), rabbit beta-globin polyA signal, Phage P1 loxP site, mouse Htr5b genomic DNA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. [Htr5B-tTA KI: (Cell Rep., (2012).), PLP-tTA Tg: Genesis 2011 Sep 22. doi:10. 1002/dvg. 20808.] The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data