Information1 | |
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Image | |
BRC No. | RBRC05445 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Htr5b<tm1(tTA)Rhn> |
Former Common name | Htr5B-tTA KI, B6-Htr5b/tTA KI |
H-2 Haplotype | |
ES Cell line | CSL3[129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. The mutant mice were crossed to C57BL/6J over 6 times. |
Strain description | Htr5B-tTA knock-in mice. The tetracycline-controlled transactivator (tTA) gene was inserted into the Htr5b gene. tTA expression is detected in medial habenular and inferior olivary nuclei, and hippocampus CA1 region. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Genesis, 50(5):424-8 (2012). 21953949 Hippocampus 33296119 Cell Rep., 2(2):397-406 (2012). 22854021 Nat. Neurosci., 22(5):770-777 (2019). 30988523 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA1HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)1tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain |
Information2 | |
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Donor DNA | tTA(E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), rabbit beta-globin polyA signal, Phage P1 loxP site, mouse Htr5b genomic DNA |
Research application | Cre/loxP system Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. [Htr5B-tTA KI: (Cell Rep., (2012).), PLP-tTA Tg: Genesis 2011 Sep 22. doi:10. 1002/dvg. 20808.] The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper. |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |