RBRC05443

Information1

Image
BRC No.RBRC05443
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Sec23ip<tm1Katn>
Former Common nameB6-Sec23ip floxed
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsuko Tani, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences in 2008. Targeted construct was electroporated into TT2 ES cells. The mutant mice were crossed with C57BL/6 over 5 times.
Strain descriptionSec23ip, Sec23 interacting protein (p125) floxed mice. A loxP site was inserted upstream of exon 1 and a PGK-neo cassette and loxP site were inserted downstream of exon 1. Homozygous mutant mice show no obvious abnormality. Conditional Sec23ip deficient mice can be generated by crossing with tissue-specific Cre mice. Sec23ip knockout mice (RBRC05444 B6;B6CB-Sec23ip<tm1.1Katn>).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesFEBS Lett., 585, 2171-2176 (2011). 21640725

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sec23ipSec23 interacting protein7Sec23ip<tm1Katn>targeted mutation 1, Katsuko Tani

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
bovine polyA7

Information2

Donor DNAP1 phage loxP sites, 酵母菌 FRT, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter, E. coli neo, ウシ polyA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 585(14): 2171-6 (2011).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning of the BIOLOGICAL RESOURCE is published, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKatsuko Tani (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data