RBRC05440

Information1

Image
BRC No.RBRC05440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Muc21<tm2(Venus)Tats>/2
Former Common nameC57BL/6J-Muc21<tm2(Venus)Tats>/2TatsRbrc
H-2 Haplotype
ES Cell lineB6J-S1<UTR> [C57BL/6J]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tatsuro Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2011. C57BL/6J background. Main line (RBRC05439), Subline (RBRC05440).
Strain descriptionMucin 21/epiglycanin venus knock-in mice. A PGK-neo cassette and venus gene was inserted into Muc21 gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJJcl]
ReferencesGlycobiology, 18, 74-83 (2008). 17977904
J. Biol. Chem., 285, 21233-21240 (2010). 20388707

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gm9573predicted gene 957317Gm9573<tm2(Venus)Tats>targeted mutation 2, Tatsuro Irimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)17Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17

Information2

Donor DNAMouse Muc21, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, PhageP1 loxP, Yeast FRT, Aequorea victoria Venus
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTatsuro Irimura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP

BRC mice in Publications

No Data