RBRC05438

Information1

Image
BRC No.RBRC05438
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Fad104<tm1Maim>/9Maim
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Graduate School of Pharmaceutical Sciences, Nagoya City University and Genome Information Research Center, Osaka University in 2006. C57BL/6J background. Main line (RBRC05437, line 3), Subline (RBRC05438, line 9).
Strain descriptionFad104, factor for adipocyte differentiation 104 (Fndc3b, fibronectin type III domain containing 3B) gene knockout mice. Fad104 is a protein containing 9 repeats of the fibronectin type III domain and a transmembrane domain that transiently expresses at the early stage of adipocyte differentiation. Homozygous mutant mice are die within 1 day after birth. Analyses of MEFs from mutant mice revealed that disruption of this gene caused a reduction in the ability to differentiate, proliferate, adhere, spread and migrate. Heterozygous mutant mice show a less body weight gain than wild-types. Exon 2 was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote X Wild-type [C57BL/6J(Crlj)]
ReferencesBiochem. Biophys. Res. Commun., 397, 187-191 (2010). 20493170
Exp. Cell. Res., 317, 2110-2123 (2011). 21704616
Exp. Cell. Res., 315, 809-819 (2009). 19138685
FEBS Lett., 577, 49-54 (2004). 15527760

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fndc3bfibronectin type III domain containing 3B3Fndc3b<tm1Maim>targeted mutation 1, Masayoshi Imagawa

Information2

Donor DNAE. coli Neomysin resistance gene, mouse PGK promoter, HSV HSV-tk, mouse PGK polyA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Cell. Res., 315, 809-819 (2009). Exp. Cell. Res., 317, 2110-2123 (2011). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr.Makoto Nishizuka (nishizuka@hirosaki-u.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayoshi Imagawa (Nagoya City University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data