Strain Data Sheet

RBRC05432

Information1

Image
BRC No.RBRC05432
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-D1Pas1<tm1(KOMP)Vlcg>/D4
Former Common nameB6-D1Pas1 KO#D4
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/11/16Room:3-DRack:D
2020/08/24Room:3-DRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
D1Pas1DNA segment, Chr 1, Pasteur Institute 11D1Pas1<tm1(KOMP)Vlcg>targeted mutation 1, Velocigene

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1

Information2

Donor DNAVelocigene cassette ZEN-Ub1(E. coli lacZ, SV40 polyA, Phage P1 loxP sites, human ubiquitin C promoter, em7 promoter, E. coli neo, mouse Pgk1 polyA), mouse D1Pas1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Inoue H, Ogonuki N, Hirose M, Hatanaka Y, Matoba S, Chuma S, Kobayashi K, Wakana S, Noguchi J, Inoue K, Tanemura K, Ogura A.
Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
Biochem Biophys Res Commun 478(2) 592-8(2016) 27473657