RBRC05431

Information1

Image
BRC No.RBRC05431
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Ano5<tm1Itak>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain descriptionAno5, anoctamin 5 (Gdd1) knockout mice. The first exon of Ano5 gene was replaced with a neomycin selection cassette.Homozygous mutant mice show no obvious abnormality.
Colony maintenance
ReferencesAm. J. Hum. Genet., 86, 213-221 (2010). 20096397
J. Bone Miner. Res., 18, 413-418 (2003). 12619924
Biochem. Biophys. Res. Commun., 357, 126-132 (2007). 17418107
Am. J. Hum. Genet., 74, 1255-1261 (2004). 15124103
Biochem. Biophys. Res. Commun., 331, 1099-1106 (2005). 15882990

Health Report

Examination Date / Room / Rack2013/03/25Room:4-ARack:D
2013/01/21Room:4-ARack:D
2012/12/19Room:4-ARack:D
2012/12/17Room:4-ARack:D
2012/11/26Room:4-ARack:D
2012/11/26Room:4-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ano5anoctamin 57Ano5

Information2

Donor DNAmouse GDD1(ANO5)genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli. neo
Research applicationMouse Models for Human Disease
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMitsuo Itakura (Tokushima University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Xu J, El Refaey M, Xu L, Zhao L, Gao Y, Floyd K, Karaze T, Janssen PM, Han R.
Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy.
Skelet Muscle 5 43(2015) 26693275