Strain Data Sheet

RBRC05431

Information1

Image
BRC No.RBRC05431
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Ano5<tm1Itak>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hiroshi Inoue, Satoshi Tsutsumi, Mitsuo Itakura, Institute for Genome Research, The University of Tokushima and Kuniko Mizuta, Hiroshima University Graduate School of Biomedical Sciences. A neomycin selection cassette was transferred into C57BL/6 derived ES cells to replace exon 1 of Ano5 gene.
Strain descriptionAno5, anoctamin 5 (Gdd1) knockout mice. The first exon of Ano5 gene was replaced with a neomycin selection cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenance
ReferencesAm. J. Hum. Genet., 74, 1255-1261 (2004). 15124103
Biochem. Biophys. Res. Commun., 331, 1099-1106 (2005). 15882990
J. Bone Miner. Res., 18, 413-418 (2003). 12619924
Am. J. Hum. Genet., 86, 213-221 (2010). 20096397
Biochem. Biophys. Res. Commun., 357, 126-132 (2007). 17418107

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ano5anoctamin 57Ano5 <tm1Itak>targeted mutation 1, Mitsuo Itakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli. neo, mouse GDD1 (ANO5) genomic DNA
Research applicationMouse Models for Human Disease
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMitsuo Itakura (Tokushima University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Xu J, El Refaey M, Xu L, Zhao L, Gao Y, Floyd K, Karaze T, Janssen PM, Han R.
Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy.
Skelet Muscle 5 43(2015) 26693275