RBRC05429

Information1

Image
BRC No.RBRC05429
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBXSB.B6-Fcer1g<tm1Tks>/Hros
Former Common nameBXSB-Fcer1g KO BXSB. gamma-/-
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Sachiko Hirose, Department of Internal Medicine, Juntendo University School of Medicine in 2006. A neomycin selection cassette was transferred into BL/6-III ES cells to delete and replace exon 2 of Fcer1g gene. The mutant mice were backcrossed BXSB strain over 20 generations.
Strain descriptionIgG Fc receptor common gamma knockout mice. BXSB background. Exon 2 of Fcer1g gene was deleted and replaced with a neomycin selection cassette. Autoimmune disease features of male were suppressed in homozygous mutant mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to BXSB/MpJJmsSlc (female)]
ReferencesJ. Immunol., 177, 1646-1654 (2006). 16849473
J. Clin. Invest., 102, 1229-1238 (1998). 9739057

Health Report

Examination Date / Room / Rack2018/06/18Room:3-CRack:L
2018/04/16Room:3-CRack:L
2018/02/19Room:3-CRack:L
2017/12/18Room:3-CRack:L
2017/10/16Room:3-CRack:L
2017/08/21Room:3-CRack:L
2017/06/19Room:3-CRack:L
2017/04/17Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fcer1gFc receptor, IgE, high affinity I, gamma polypeptide1Fcer1g<tm1Tks>targeted mutation 1, Takashi Saito

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse fcer1g genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 177, 1646-1654 (2006).
DepositorSachiko Hirose (Juntendo University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data