RBRC05428

Information1

Image
BRC No.RBRC05428
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Engase<tm1Tasuz>
Former Common name
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS Genet., 13(4):e1006696 (2017). 28426790
Proc. Natl. Acad. Sci. USA, 112, 1398-1403 (2015). 25605922

Health Report

Examination Date / Room / Rack2019/01/28Room:4-ARack:G
2018/11/26Room:4-ARack:G
2018/09/25Room:4-ARack:G
2018/07/30Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Engaseendo-beta-N-acetylglucosaminidase11Engase<tm1Tasuz>targeted mutation 1, Tadashi Suzuki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 poly A, mouse Engase genomic DNA [The SV40 Poly A signal was not detected by PCR.]
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. 2017 Apr 20;13(4):e1006696. doi: 10.1371/journal.pgen.1006696. eCollection 2017 Apr.
DepositorTadashi Suzuki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data