RBRC05390

Information1

Image
BRC No.RBRC05390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Sept7<tm1Ksh>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Makoto Kinoshita, Nagoya University Graduate School of Science in 2007-8. C57BL/6 background. A frt flanked neo cassette was removed by FRP meditated recombination.
Strain descriptionSept7 floxed mice. Exon 3 of Sept7 gene was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesNat Commun. 2013;4:2532. doi: 10.1038/ncomms3532 24113571
PLoS Genetics 10(8): e1004558, 2014. 25122120

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sept7septin 79Sept7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Information2

Donor DNAE. coli Neomycin resistance gene, P1 phage loxP, Yeast FRT (flippase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2013;4:2532. doi: 10.1038/ncomms3532In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMakoto Kinoshita (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data