Strain Data Sheet

RBRC05390

Strain Information

Image
BRC No.RBRC05390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Sept7<tm1Ksh>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Makoto Kinoshita, Nagoya University Graduate School of Science in 2007-8. C57BL/6 background. A frt flanked neo cassette was removed by FRP meditated recombination.
Strain descriptionSept7 floxed mice. Exon 3 of Sept7 gene was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References
Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation.
Ageta-Ishihara N, Miyata T, Ohshima C, Watanabe M, Sato Y, Hamamura Y, Higashiyama T, Mazitschek R, Bito H, Kinoshita M
Nat Commun. 2013;4:2532. doi: 10.1038/ncomms3532 24113571

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.
Menon M B, Sawada A, Chaturvedi A, Mishra P, Schuster-Gossler K, Galla M, Schambach A, Gossler A, Forster R, Heuser M, Kotlyarov A, Kinoshita M, Gaestel M
PLoS Genetics 10(8): e1004558, 2014. 25122120

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site9Frt
Sept7septin 79Sept7
loxPphage P1 loxP9loxP
loxPphage P1 loxP9loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal axon morphology(MP:0005404)

  • abnormal dendrite morphology(MP:0008143)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, P1 phage loxP, Yeast FRT (flippase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2013;4:2532. doi: 10.1038/ncomms3532In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorMakoto Kinoshita (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data