Strain Data Sheet

RBRC05389

Strain Information

Image
BRC No.RBRC05389
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Capsl<tm1Hmd>
Former Common nameB6-Caps1 KI/lacZ
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Yanick Botilde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006.
Strain descriptionCapsl IRES-lacZ knock-in mice. LacZ expresses in node at 8-cell embryos.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Capslcalcyphosine-like15Capsl targeted mutation 1, Hiroshi Hamada
Frtyeast FRT (flippase recombination target) site15Frt
IRESinternal ribosomal entry site (EMCV)15
lacZbeta-galactosidase (E. coli)15
loxPphage P1 loxP15loxP
neoneomycin resistance gene (Klebsiella pneumoniae)15mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, yeast frt site, E. coli LacZ gene, synthetic bacterial EM7 promoter (T7 phage), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Klebsiella pneumoniae neo, mouse PGK promoter
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data