RBRC05388

Information1

Image
BRC No.RBRC05388
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3-Tg(Wnt3-lacZ)5Hmd
Former Common nameB6C3-Wnt3-lacZ Tg#5
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The transgene was microinjected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionAn lacZ reporter gene was inserted into Wnt3 BAC clone at the initiating ATG codon. Expression of the transgene matches that of the endogenous Wnt3 at 8-cell mouse embryos.
Colony maintenanceCarrier x Noncarrier [or Crossing to B6C3F1]
ReferencesNat Commun. 2012;3:1322. doi 23271656

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNmouse Wnt3 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA additional signalUN

Information2

Donor DNAE.coli LacZ, SV40 Poly A additional signal, mouse Wnt3 promoter
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data